Abstract

Background/Objectives: Aim of this works of researches proposed the detection of the isoform p53 protein in the patients with Chronic Lymphocytic Leukemia of type B, (CLL-B), performed using the quantitative sandwich Enzyme linked immunoassay ELISA for the direct detection of the p53 isoform protein, a product of the mutant P53 gene. Methods: Additionally, the cellular energy levels, particularly in CLL-B, were measured using a bioluminescence method. The study employed a quantitative sandwich ELISA method for the direct detection of the p53 isoform protein, a product of the mutant P53 gene Adenosine triphosphate, (ATP), was measured using the standard bioluminescence principle on an automatic LKB analyzer with an ATP monitoring reagent. Results: The presence of isoform p53 protein in the studied group was calculated at 17%. Unfavorable evolution and transformation of CLL into Diffuse Large B-Cell Lymphoma (DLBCL) were observed in 3.5% of the cases. In such as malignant diseases, increased ATP values were observed in Chronic Lymphatic Leukemia in values of 4.30-4.55 μM ATP, (Normal energy in B Cells: x̄ = 0.35 μM ATP, (SD = 0.42). Conclusion: Maturation state of the clonal tumor and the prognosis of patients with CLL depend on anaerobic metabolism and the expression of the p53 protein isoform.

Keywords: chronic lymphocytic leukemia, P53 gene, isoform p53 protein, CD-5 receptor, Diffuse Large Lymphoma, adenosine triphosphate

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How to Cite
Udristioiu, A., Giubelan, A., Velcea, A., Martin, L., Cojocaru, M., & Gheorghe, I.-O. (2025). Measurements of p53 Isoform Protein Concentration and Energetic Levels in Cells of CLL Using Enzymatic Methods. International Journal of Innovative Research in Medical Science, 10(05), 179–185. https://doi.org/10.23958/ijirms/vol10-i05/2073

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Copyright © 2025 Aurelian Udriştioiu Alexandru Giubelan Ana Velcea Liviu Martin Manole Cojocaru Ioan-Ovidiu Gheorghe this is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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